发明授权
US06403326B1 Methods of using antibodies against human luteinizing hormone-related determinants to detect and enrich human malignant cells
失效
使用针对人黄体生成激素相关决定簇的抗体来检测和富集人类恶性细胞的方法
- 专利标题： Methods of using antibodies against human luteinizing hormone-related determinants to detect and enrich human malignant cells
- 专利标题（中）： 使用针对人黄体生成激素相关决定簇的抗体来检测和富集人类恶性细胞的方法
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申请号： US08478431申请日： 1995-06-07
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公开(公告)号： US06403326B1公开(公告)日： 2002-06-11
- 发明人: Hernan Acevedo , Alexander Krichevsky
- 申请人： Hernan Acevedo , Alexander Krichevsky
- 主分类号： G01N723
- IPC分类号： G01N723
摘要：
The subject invention provides a method for detecting the presence of human malignant cells in a sample of tumor cells, which comprises contacting the sample with an antibody directed to an epitope present on the &bgr; subunit of human luteinizing hormone or on intact human luteinizing hormone under conditions such that the antibody forms a complex with cells present in the sample if the epitope is present on the surface of the cells, and determining whether the antibody forms such a complex. The subject invention also provides a method for determining whether a tumor present in a human subject is malignant which comprises obtaining a sample of cells from the tumor and detecting the presence of malignant cells in the sample according to the method of the subject invention. Finally, the subject invention provides a method for obtaining an enriched population of live human malignant cells which comprises contacting a population of cells comprising live human malignant cells with an antibody directed to an epitope present on the &bgr; subunit of human luteinizing hormone or on intact human luteinizing hormone under conditions such that the antibody forms a complex with the cells present in the population if the epitope is present on the surface of the cells, and isolating the cells which form a complex with the antibody.