发明授权
- 专利标题： Combinatorial selection of oligonucleotide aptamers
- 专利标题（中）： 组合选择寡核苷酸适体
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申请号： US09425798申请日： 1999-10-25
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公开(公告)号： US06423493B1公开(公告)日： 2002-07-23
- 发明人: David G. Gorenstein , David J. King , Daniel A. Ventura , Allan R. Brasier
- 申请人： David G. Gorenstein , David J. King , Daniel A. Ventura , Allan R. Brasier
- 主分类号： C12Q168
- IPC分类号： C12Q168
摘要：
A random combinatorial selection method is disclosed for the construction of oligonucleotide aptamers in which nuclease resistance is conferred by the inclusion of modified nucleotides. The modified nucleotides are incorporated during PCR amplification to form achiral modified oligonucleotides. Thio-substituted aptamers are provided that bind tightly to the nuclear factor for human IL6 (NF-IL6).
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