发明授权
US06436675B1 Use of codon-varied oligonucleotide synthesis for synthetic shuffling
失效
使用密码子变化的寡核苷酸合成进行合成洗牌
- 专利标题： Use of codon-varied oligonucleotide synthesis for synthetic shuffling
- 专利标题（中）： 使用密码子变化的寡核苷酸合成进行合成洗牌
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申请号： US09408393申请日： 1999-09-28
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公开(公告)号： US06436675B1公开(公告)日： 2002-08-20
- 发明人: Mark Welch , Jon Ness , Claes Gustafsson , Willem P. C. Stemmer , Jeremy Minshull
- 申请人： Mark Welch , Jon Ness , Claes Gustafsson , Willem P. C. Stemmer , Jeremy Minshull
- 主分类号： C12Q168
- IPC分类号： C12Q168
摘要：
Methods of providing shuffling libraries that include codon-varied oligonucleotide sequences are described. Codon-varied oligonucleotides are synthesized using trinucleotide or mononucleotide phosphoramidite sequences, and are derived from homologous or non-homologous nucleic acid sequences, or combinations of such sequences. Various methods of recombining codon-varied oligonucleotide sequences to expedite artificial evolution are also described. The present invention additionally relates to various integrated systems that are optionally used to automate these recombination methods.
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