发明授权
US06475720B1 Chromosome-specific staining to detect genetic rearrangements associated with chromosome 3 and/or chromosome 17
失效
染色体特异性染色以检测与染色体3和/或染色体17相关的遗传重排
- 专利标题: Chromosome-specific staining to detect genetic rearrangements associated with chromosome 3 and/or chromosome 17
- 专利标题(中): 染色体特异性染色以检测与染色体3和/或染色体17相关的遗传重排
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申请号: US08478387申请日: 1995-06-07
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公开(公告)号: US06475720B1公开(公告)日: 2002-11-05
- 发明人: Joe W. Gray , Daniel Pinkel , Olli-Pekka Kallioniemi , Anne Kallioniemi , Masaru Sakamoto
- 申请人: Joe W. Gray , Daniel Pinkel , Olli-Pekka Kallioniemi , Anne Kallioniemi , Masaru Sakamoto
- 主分类号: C12Q168
- IPC分类号: C12Q168
摘要:
Methods and compositions for staining based upon nucleic acid sequence that employ nudeic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML), retinoblastoma, ovarian and uterine cancers, and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.