发明授权
- 专利标题: Multiply-substituted protease variants
- 专利标题(中): 多重取代的蛋白酶变体
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申请号: US09178173申请日: 1998-10-23
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公开(公告)号: US06482628B1公开(公告)日: 2002-11-19
- 发明人: Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell , Andre C. Baeck
- 申请人: Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell , Andre C. Baeck
- 主分类号: C12N956
- IPC分类号: C12N956
摘要:
Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.