- 专利标题: Method for the detection of an analyte by means of a nucleic acid reporter
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申请号: US09858994申请日: 2001-05-16
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公开(公告)号: US06511809B2公开(公告)日: 2003-01-28
- 发明人: Luis Baez , Richard C. Ebersole , Edwin R. Hendrickson , Neel Neelkantan , Michael P. Perry
- 申请人: Luis Baez , Richard C. Ebersole , Edwin R. Hendrickson , Neel Neelkantan , Michael P. Perry
- 主分类号: C12Q168
- IPC分类号: C12Q168
摘要:
A process is disclosed for the detection of an analyte utilizing a nucleic acid label as a reporter. The analyte is detected by the binding of at least two reporter conjugates, each conjugate comprising a member of a binding pair and a nucleic acid label. The binding of the reporter conjugates to the analyte facilitates the juxtaposition of the nucleic acid labels, forming a single nucleic acid amplicon. The amplicon may then be detected directly, or may be used as a template of the generation of amplification products. Detection of the analyte by this process significantly reduces assay background caused by non-specific reporter conjugate binding.
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