A cartridge positioning and holding mechanism has a fixed guide for positioning a single-open-ended cartridge when the single-open-ended cartridge is inserted in an axial direction thereof with a cap positioned ahead, and a movable chuck openable and closable for forming and holding the single-open-ended cartridge after a film coil is inserted into the single-open-ended cartridge in an axial direction of the spool of the film coil. The film coil can be inserted into the single-open-ended cartridge highly accurately and efficiently with a simple and small arrangement.