发明授权
- 专利标题: Cryogenic preservation of biologically active material using high temperature freezing
- 专利标题(中): 使用高温冷冻对生物活性物质进行低温保存
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申请号: US09655807申请日: 2000-09-06
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公开(公告)号: US06519954B1公开(公告)日: 2003-02-18
- 发明人: Samuel D. Prien , John Blanton , Kevin R. Pond , Markus F. Miller , Brian Wood , Allan J. Cassell
- 申请人: Samuel D. Prien , John Blanton , Kevin R. Pond , Markus F. Miller , Brian Wood , Allan J. Cassell
- 主分类号: F17B1702
- IPC分类号: F17B1702
摘要:
Viable biological material is cryogenically preserved (cryopreservation) by preparing the material for freezing, immersing the material in a tank of cooling fluid, and circulating the cooling fluid past the material at a substantially constant predetermined velocity and temperature to freeze the material. A method according to the present invention freezes the biologic material quickly enough to avoid the formation of ice crystals within cell structures (vitrification). The temperature of the cooling fluid is preferably between −20° C. and −30° C., which is warm enough to minimize the formation of stress fractures in cell membranes due to thermal changes. Cells frozen using a method according to the present invention have been shown to have approximately an 80 percent survival rate, which is significantly higher than other cryopreservation methods.
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