A wipe inspecting instrument comprising a sample collecting member and a reactive reagent container. The sample collecting member comprises a tubular main body having both upper and lower ends thereof opened, a sample wiping member removably fitted to the upper open portion of the main body and an extracting liquid container disposed below the main body. The sample wiping member includes a retaining member inserted into the upper part of the main body and a cotton swab held by the retaining member. Further, the sample collecting member includes a splash-proofing member disposed at a lower part of the extracting liquid container, for preventing the splash of the extracting liquid. The reactive reagent container has a test tube shape and so inserted into the open portion at the lower end of the main body as to be capable of moving up and down.