Use of betel leaf extract to induce IFN-gamma production from human peripheral blood T cells and as a Th1 type immunomodulator

Invention Grant

US06531166B2 Use of betel leaf extract to induce IFN-gamma production from human peripheral blood T cells and as a Th1 type immunomodulator 失效

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Patent Title: Use of betel leaf extract to induce IFN-gamma production from human peripheral blood T cells and as a Th1 type immunomodulator
Application No.: US09746017

Application Date: 2000-12-26
Publication No.: US06531166B2

Publication Date: 2003-03-11
Inventor: Santu Bandyopadhyay , Bikash Pal , Samir Bhattacharya , Mitali Ray , Keshab Chandra Roy
Applicant: Santu Bandyopadhyay , Bikash Pal , Samir Bhattacharya , Mitali Ray , Keshab Chandra Roy
Main IPC: A61K3578
IPC: A61K3578

Use of betel leaf extract to induce IFN-gamma production from human peripheral blood T cells and as a Th1 type immunomodulator

Abstract:

This invention relates to use of betel leaf extract to induce IFN&ggr; from human peripheral blood mononuclear cells and as a Th1 type immuno modulator, wherein the said method comprises the steps of, preparing water extract of betel leaf, preparation of human peripheral blood mononuclear cells, incubation of hPBMC with betel leaf extract for a period of 18-48 hours, extraction of RNA for cytokine specific RT-PCR or for flow cytometry for the detection of intracellular cytokine protein, subjecting RNA for RT-PCR to obtain PCR products using IFN&ggr; specific known primers, and enhancement of IFN&ggr; as reflected by IFN&ggr; specific band.

Public/Granted literature

US20020146470A1 Use of betel leaf extract to induce IFN-gamma production from human peripheral blood T cells and as a Th1 type immunomodulator Public/Granted day:2002-10-10

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