Process for isolating glycomacropeptide from dairy products with a phenylalanine impurity of 0.5% w/w

发明授权

US06555659B1 Process for isolating glycomacropeptide from dairy products with a phenylalanine impurity of 0.5% w/w 失效

标题翻译：从具有0.5％w / w的苯丙氨酸杂质的乳制品中分离糖巨肽的方法

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专利标题： Process for isolating glycomacropeptide from dairy products with a phenylalanine impurity of 0.5% w/w
专利标题（中）： 从具有0.5％w / w的苯丙氨酸杂质的乳制品中分离糖巨肽的方法
申请号： US09625043

申请日： 2000-07-24
公开(公告)号： US06555659B1

公开(公告)日： 2003-04-29
发明人: John Stephen Ayers , Kay Patricia Coolbear , David Francis Elgar , Mark Pritchard
申请人： John Stephen Ayers , Kay Patricia Coolbear , David Francis Elgar , Mark Pritchard
优先权： NZ299483 19961001
主分类号： C07K1400
IPC分类号： C07K1400

Process for isolating glycomacropeptide from dairy products with a phenylalanine impurity of 0.5% w/w

摘要：

The present invention is related to a method for the purification of glycomacropeptide (GMP) with an amino acid composition containing no greater that 0.5% (w/w) phenylalanine, comprising the steps of contacting a GMP-containing feedstock with a first anion exchanger under conditions to adsorb the GMP, eluting the adsorbed GMP from the anion exchanger and removing impurities from the GMP-containing eluate by either: (i) contacting the GMP-containing eluate with a cation exchanger in conditions under which the impurities in the eluate are adsorbed onto the cation exchanger, or (ii) precipitating the impurities in GMP-containing eluate using conditions in which the GMP remains i solution, or (iii) contacting the GMP-containing eluate with a second anion exchanger in conditions under which the impurities in the eluate are adsorbed onto the anion exchanger, and recovering the GMP from whichever one or more of the steps (i), (ii) or (iii) was used.

摘要（中）：

本发明涉及一种纯化含有不大于0.5％（w / w）苯丙氨酸的氨基酸组成的糖巨肽（GMP）的方法，该方法包括以下步骤：使含GMP的原料与第一阴离子交换剂接触吸附GMP的条件，从阴离子交换器洗脱吸附的GMP并从含GMP的洗脱液中除去杂质，方法是：（i）在吸附洗脱液中的杂质的条件下将含GMP的洗脱液与阳离子交换剂接触或者（ii）使用GMP保留在溶液中的条件沉淀含GMP的洗脱液中的杂质，或者（iii）使含GMP的洗脱液与第二阴离子交换剂接触，洗脱液被吸附到阴离子交换器上，并且从使用步骤（i），（ii）或（iii）中的一个或多个步骤回收GMP。

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