发明授权
US06566128B1 Adenovirus vectors generated from helper viruses and helper-dependent vectors
失效
由辅助病毒和辅助因子载体产生的腺病毒载体
- 专利标题： Adenovirus vectors generated from helper viruses and helper-dependent vectors
- 专利标题（中）： 由辅助病毒和辅助因子载体产生的腺病毒载体
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申请号： US09440809申请日： 1999-11-15
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公开(公告)号： US06566128B1公开(公告)日： 2003-05-20
- 发明人: Frank L. Graham , Robin Parks , Liane Chen
- 申请人： Frank L. Graham , Robin Parks , Liane Chen
- 主分类号： A61K4800
- IPC分类号： A61K4800
摘要：
The present invention provides an improved helper-dependent vector system for production of high capacity adenoviral cloning vectors. The invention makes use of the DNA size packaging constraints imposed on a pIX-defective Ad virion that prevent such virions from packaging DNA larger than approximately 35 kb. This constraint can be used to develop helper viruses that do not package their DNA. In one embodiment, the invention combines this methodology with the Cre-loxP helper-dependent system to decrease the quantity of contaminating helper virus in vector preparations. In another embodiment the invention is used for vector growth.
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