发明授权
US06566587B1 Mutated 5-enolpyruvylshikimate-3-phosphate synthase, gene coding for said protein and transformed plants containing said gene
失效
突变的5-烯醇丙酮酸莽草酸-3-磷酸合酶，编码所述蛋白质的基因和含有所述基因的转化植物
- 专利标题： Mutated 5-enolpyruvylshikimate-3-phosphate synthase, gene coding for said protein and transformed plants containing said gene
- 专利标题（中）： 突变的5-烯醇丙酮酸莽草酸-3-磷酸合酶，编码所述蛋白质的基因和含有所述基因的转化植物
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申请号： US08945144申请日： 1998-01-20
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公开(公告)号： US06566587B1公开(公告)日： 2003-05-20
- 发明人: Michel Lebrun , Alain Sailland , Georges Freyssinet , Eric Degryse
- 申请人： Michel Lebrun , Alain Sailland , Georges Freyssinet , Eric Degryse
- 优先权： FR9508979 19950719
- 主分类号： C12N1582
- IPC分类号： C12N1582
摘要：
The present invention relates to DNA molecules of plant origin encoding a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme wherein the a first EPSPS coding sequence that normally encodes a threonine residue of a mature EPSPS sequence is modified to encode isoleucine of the mature EPSPS sequence, and a second EPSPS coding sequence that normally encodes a proline residue of a mature EPSPS sequence is modified to encode serine of the mature EPSPS sequence, wherein the first and second residues are respectively located at relatively positions 102 and 106 of a mature EPSPS sequence encoded by said DNA molecule; and the production of a transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The mutated enzyme, which substantially maintains the catalytic activity of the wild-type enzyme, allows for increased tolerance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells.
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