In the present invention, a transmission line length estimation circuit with continuous feedback equalizer is provided. The transmission line length estimation circuit comprises a DC bias circuit and a peak detector circuit to generate a DC voltage according to a different cable length. An equalizer core circuit is for receiving the first signal and generating a second signal. A peak detector circuit is used for the first signal and the second signal. A transmission line length detector circuit is coupled to the peak detector circuit and used for generating a plurality of first parameters for phase shift and amplitude losses according to the different cable length. An internal pattern calibration circuit is multiplexed to the first signal and used for generating a plurality of second parameters for calibration of close loop. A feedback control circuit is connected to the equalizer core circuit and used for continuously fine tuning of the equalizer core circuit according to the first parameters and the second parameters.