Heterodimeric nuclear receptors proteins, genes encoding same, and usage thereof

发明授权

US06635429B1 Heterodimeric nuclear receptors proteins, genes encoding same, and usage thereof 失效

标题翻译：异二聚体核受体蛋白，编码相同的基因及其用途

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专利标题： Heterodimeric nuclear receptors proteins, genes encoding same, and usage thereof
专利标题（中）： 异二聚体核受体蛋白，编码相同的基因及其用途
申请号： US08216592

申请日： 1994-03-23
公开(公告)号： US06635429B1

公开(公告)日： 2003-10-21
发明人: Mark Leid , Philippe Kastner , Pierre Chambon
申请人： Mark Leid , Philippe Kastner , Pierre Chambon
主分类号： G01N3353
IPC分类号： G01N3353

Heterodimeric nuclear receptors proteins, genes encoding same, and usage thereof

摘要：

The present invention is based in part on the novel observation that two different types of nuclear receptors, retinoic acid receptors (RAR) and thyroid receptors (TR) dimerize with a RX receptor (RXR) to form a heterodimer which is capable of binding to retinoic acid response elements (RARE) or thyroid receptor response elements (TRE) at physiological conditions. In addition, the present invention discloses that RXR homodimers are capable of binding to RARE's. Based on this observation, the present invention provides novel dimeric proteins, methods of identifying agents capable of binding the dimers of the present invention, methods of identifying DNA sequences capable of being bound by the dimers and methods to identify RA metabolic enzymes and proteins which are required for the activation function of nuclear receptors.

摘要（中）：

本发明部分基于两种不同类型的核受体，视黄酸受体（RAR）和甲状腺受体（TR）与RX受体（RXR）二聚化以形成能够结合视黄酸的异源二聚体的新观察酸反应元素（RARE）或甲状腺受体反应元件（TRE）。另外，本发明公开了RXR同型二聚体能够与RARE结合。基于该观察，本发明提供了新颖的二聚蛋白，鉴定能够结合本发明的二聚体的试剂的方法，鉴定能够被二聚体结合的DNA序列的方法以及鉴定RA代谢酶和蛋白质的方法核受体激活功能所需。

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