发明授权
- 专利标题： High-sensitive detection of environmental pollutants
- 专利标题（中）： 环境污染物高敏感检测
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申请号： US10094151申请日： 2002-03-11
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公开(公告)号： US06740521B2公开(公告)日： 2004-05-25
- 发明人: Hiroko Isoda , Takashi Koyama , Masako Tasaki , Syuichi Oka , Norio Sugiura , Takaaki Maekawa , Yuhei Inamori , Shinichi Yokota , Mikio Kitahara , Kazuhiro Nagata
- 申请人： Hiroko Isoda , Takashi Koyama , Masako Tasaki , Syuichi Oka , Norio Sugiura , Takaaki Maekawa , Yuhei Inamori , Shinichi Yokota , Mikio Kitahara , Kazuhiro Nagata
- 优先权： JP2001-272478 20010907
- 主分类号： C12N500
- IPC分类号： C12N500
摘要：
The present invention provides a bioassay system, for detection of hazardous chemical substances, natural toxic substances in the environment and unknown toxic compounds, with high sensitivity, simplicity and speed. The invention provides cells and a method of using the cells for use in the bioassay system. The cell provided by the invention contains a heat shock factor binding DNA sequence and a transcriptional regulatory sequence necessary on an occasion of stress induction as a transcriptional regulatory factor binding site. The cell also possesses a reporter gene under the control of the promoter. The reporter gene is connected, on the downstream side, to the SV40pA signal without any intervening intron.
公开/授权文献
- US20030049737A1 High-sensitive detection of environmental pollutants 公开/授权日：2003-03-13