发明授权
US06764843B2 Method of cloning and expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli and purification of BsmBI endonuclease
有权
在大肠杆菌中克隆和表达BsmBI限制性内切核酸酶和BsmBI甲基化酶的方法，并纯化BsmBI内切核酸酶
- 专利标题： Method of cloning and expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli and purification of BsmBI endonuclease
- 专利标题（中）： 在大肠杆菌中克隆和表达BsmBI限制性内切核酸酶和BsmBI甲基化酶的方法，并纯化BsmBI内切核酸酶
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申请号： US09966997申请日： 2001-09-28
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公开(公告)号： US06764843B2公开(公告)日： 2004-07-20
- 发明人: Shuang-yong Xu , Andrew Dore , Adam Hume , John Pelletier , Jing Zhou
- 申请人： Shuang-yong Xu , Andrew Dore , Adam Hume , John Pelletier , Jing Zhou
- 主分类号： C12N922
- IPC分类号： C12N922
摘要：
The present invention relates to recombinant DNA that encodes the BsmBI restriction endonuclease as well as BsmBI methyltransferase, expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli cells containing the recombinant DNA. It also relates to a method for purification of the recombinant BsmBI restriction endonuclease.
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