发明授权
- 专利标题: Method for producing DNA chip
- 专利标题(中): 生产DNA芯片的方法
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申请号: US10128777申请日: 2002-04-24
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公开(公告)号: US06776960B2公开(公告)日: 2004-08-17
- 发明人: Toshikazu Hirota , Nobuo Takahashi , Yukihisa Takeuchi , Takao Ohnishi
- 申请人: Toshikazu Hirota , Nobuo Takahashi , Yukihisa Takeuchi , Takao Ohnishi
- 优先权: JP11-301627 19991022; JP2000-069285 20000313
- 主分类号: G01N1506
- IPC分类号: G01N1506
摘要:
Disclosed is a method comprising a pretreatment step of forming a poly-L-lysine layer on a surface of a base plate, a sample preparation step of preparing a sample containing a DNA fragment, a dilution step of diluting the concentration of the obtained sample, and a supply step of supplying a diluted sample solution onto the base plate to produce a DNA chip. The sample preparation step includes an amplification step of PCR-amplifying the DNA fragment to prepare a PCR product, a powder formation step of drying the obtained PCR product to form DNA powder, and a mixing step of dissolving the obtained DNA powder in a buffer solution.
公开/授权文献
- US20020115101A1 Method for producing DNA chip 公开/授权日:2002-08-22