A spoke fastener includes a shank extending along a shank axis and having an anchoring portion and an operating portion. The anchoring portion is formed with resilient arm segments that are angularly spaced apart from each other relative to the shank axis. Each of the resilient arm segments has a distal end formed with a radial outward anchor projection to form the anchoring portion with an initial cross-section. The anchor projections are forcible radially and inwardly toward the shank axis to form the anchoring portion with a restricted cross-section, there by permitting extension of the anchoring portion through a spoke fastening hole in a wheel rim and retention of the anchoring portion on the wheel rim. A through hole extends through the operating and anchoring portions, permits extension of a spoke into the shank, and has a threaded segment for threaded engagement with the spoke.