发明授权
- 专利标题: Method for analyzing gene expression frequency
- 专利标题(中): 分析基因表达频率的方法
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申请号: US09926028申请日: 2001-08-16
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公开(公告)号: US06806049B1公开(公告)日: 2004-10-19
- 发明人: Takami Maekawa , Akira Mitsui , Masayo Date , Hisao Fukuda , Yoshiyuki Takahara
- 申请人: Takami Maekawa , Akira Mitsui , Masayo Date , Hisao Fukuda , Yoshiyuki Takahara
- 优先权: JP11-38538 19990217
- 主分类号: C12Q168
- IPC分类号: C12Q168
摘要:
In gene expression frequency analysis, a vector primer to which each cDNA is ligated is formed by synthesizing the cDNA from each mRNA using by a vector primer having a poly(T) sequence; each cDNA sequence is converted to a tag on the vector primer; a concatemer is formed by ligating the obtained tags via a sequence that enables recognition of ends of the tags; and the nucleotide sequence of the concatemer is analyzed.
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