发明授权
- 专利标题: Methods of screening for modifiers of potassium channel protein activity
- 专利标题(中): 筛选钾通道蛋白活性调节剂的方法
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申请号: US09965830申请日: 2001-10-01
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公开(公告)号: US06828101B2公开(公告)日: 2004-12-07
- 发明人: Akira Miyake , Shinobu Mochizuki , Hiromichi Yokoi
- 申请人: Akira Miyake , Shinobu Mochizuki , Hiromichi Yokoi
- 优先权: JPP.10-11434 19980123; JPP.10-346198 19981204
- 主分类号: C12Q168
- IPC分类号: C12Q168
摘要:
The present invention relates to a novel potassium channel protein expressed exclusively in the brain, a gene encoding this protein, a vector containing this gene, a host cell containing this vector, and so on. A typical method for obtaining the potassium channel protein of the present invention; mRNA is extracted from human cells or tissue capable of producing the potassium channel protein of the present invention. Then use is made of two primers between which the channel protein mRNA or a part of the mRNA region is located by using the thus extracted mRNA as a template. By carrying out a reverse transcriptase-polymerase chain reaction (to be referred to as RT-PCR hereinafter), the channel protein cDNA or a part thereof can be obtained. Thereafter, the channel protein can be produced by integrating the thus obtained novel potassium channel cDNA or a part of the same into an appropriate expression vector and then effecting its expression in a host cell.
公开/授权文献
- US20020177201A1 Novel postassium channel protein 公开/授权日:2002-11-28
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