发明授权
US06833143B1 Process for the preparation of a extract rich in bacosides from the herb Bacopa monniera
失效
从草药Bacopa monniera制备富含芽孢杆菌的提取物的方法
- 专利标题: Process for the preparation of a extract rich in bacosides from the herb Bacopa monniera
- 专利标题(中): 从草药Bacopa monniera制备富含芽孢杆菌的提取物的方法
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申请号: US10400192申请日: 2003-03-26
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公开(公告)号: US06833143B1公开(公告)日: 2004-12-21
- 发明人: Atul Prakash Kahol , Tarun Singh , Sudeep Tandon , Madan Mohan Gupta , Suman Preet Singh Khanuja
- 申请人: Atul Prakash Kahol , Tarun Singh , Sudeep Tandon , Madan Mohan Gupta , Suman Preet Singh Khanuja
- 主分类号: A61K3578
- IPC分类号: A61K3578
摘要:
The present invention provides a novel process for the preparation of bacosides enriched fraction in a non-hygroscopic form the extract of Bacopa monniera, the said process comprising the steps of drying freshly harvested herb in a hot air oven at 37-42° C., powdering and sieving the dried herb to obtain powder of 30-40 mesh size, defatting the powdered herb with hexane in a modified soxhlet extractor, extracting the defatted powdered herb with acetone, again extracting the same herb with methanol to obtain an extract containing bacosides, concentrating the extract to one twentieth of its original volume under reduced pressure, gradually adding the concentrated extract to acetone for precipitating the bacosides, filtering the bacosides in a Nutsche type vacuum filter, dissolving the crude bacoside mass into 2-10 parts water, extracting the bacoside solution with n-butanol to selectively transfer the bacosides to the solvent phase, separating and concentrating the solvent phase under vacuum to obtain semi-dry mass, dissolving the semi dried mass into 2-10 parts water, adding and stirring 1-5% of &bgr;-cyclodextrin to stabilize the bacosides, spray drying the stabilized bacoside solution by maintaining hot air temperature at 90-110° C., to obtain a stable free flowing fraction of Bacopa monniera rich in bacosides.
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