发明授权
- 专利标题: Expression of the toxic portion of Cry1A in plants
- 专利标题(中): Cry1A在植物中的有毒部分的表达
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申请号: US10394548申请日: 2003-03-21
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公开(公告)号: US06833449B1公开(公告)日: 2004-12-21
- 发明人: Kenneth A. Barton , Michael J. Miller
- 申请人: Kenneth A. Barton , Michael J. Miller
- 主分类号: C12N1532
- IPC分类号: C12N1532
摘要:
Disclosed is a method for improving the expression of Cry1A in plants that makes use of codons preferentially used in native plant genes. The coding sequence of the gene for the Bacillus thuringiensis delta endotoxin Cry1A crystal protein was analyzed and found to have codons not preferred by plants. By constructing a synthetic protein coding sequence that uses codons which are preferred in plant genes, expression of the protein in plant cells was improved.
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