A protective cover assembly for a cart and it's contents of the type used in hospitals and like medical facilities for the temporary storage and delivery of a plurality of items which should be maintained in a substantially sterile environment. The cover assembly comprises an enclosure formed from a flexible, preferably hypo-allergenic material dimensioned to receive substantially the entire cart therein. A plurality of reinforced portions are secured to the enclosure and are structured to prevent rupture or other physical damage thereto. A storage assembly comprises one or more compartments including at least one interior pocket disposed and structured to isolate the contents thereof.