发明授权
US06905877B1 Compositions and methods for altering amino acid content of proteins
失效
用于改变蛋白质氨基酸含量的组合物和方法
- 专利标题: Compositions and methods for altering amino acid content of proteins
- 专利标题(中): 用于改变蛋白质氨基酸含量的组合物和方法
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申请号: US09478598申请日: 2000-01-06
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公开(公告)号: US06905877B1公开(公告)日: 2005-06-14
- 发明人: A. Gururaj Rao , Heidi Major Sleister
- 申请人: A. Gururaj Rao , Heidi Major Sleister
- 申请人地址: US IA Johnston
- 专利权人: Pioneer Hi-Bred International, Inc.
- 当前专利权人: Pioneer Hi-Bred International, Inc.
- 当前专利权人地址: US IA Johnston
- 代理机构: Alston & Bird LLP
- 主分类号: A01H1/00
- IPC分类号: A01H1/00 ; A01H5/00 ; C07K14/415 ; C12N5/04 ; C12N15/10 ; C12N15/82 ; C40B40/02 ; C12N15/00
摘要:
Methods and compositions for altering amino acid composition of a protein of interest are provided, particularly proteins whose three-dimensional structure is unknown. The method comprises creating interacting molecules to the native protein and selecting for engineered proteins which retain the native conformation by antibody binding. In this manner, the levels of essential amino acids in a protein can be increased yet the biological activity of the protein maintained. Also provided is an exemplary plant protein—Glycine max vegetative storage protein (VSP)—in which methionine levels have been increased.