发明授权
- 专利标题: Oil bodies and associated proteins as affinity matrices
- 专利标题(中): 油体和相关蛋白质作为亲和基质
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申请号: US09707167申请日: 2000-11-07
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公开(公告)号: US06924363B1公开(公告)日: 2005-08-02
- 发明人: Maurice Moloney , Joseph Boothe , Gijs van Rooijen
- 申请人: Maurice Moloney , Joseph Boothe , Gijs van Rooijen
- 申请人地址: CA Calgary
- 专利权人: SemBioSys Genetics Inc.
- 当前专利权人: SemBioSys Genetics Inc.
- 当前专利权人地址: CA Calgary
- 代理机构: Bereskin & Parr Micheline Gravelle
- 主分类号: B01D15/08
- IPC分类号: B01D15/08 ; C01G11/00 ; C07H21/00 ; C07H21/04 ; C07K1/22 ; C07K14/415 ; C07K16/06 ; C07K17/02 ; C07K19/00 ; C08B1/00 ; C12N9/74 ; C12S3/14 ; A23J1/00
摘要:
A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.