- 专利标题： Method of analyzing intestinal flora and analytical apparatus
- 专利标题（中）： 肠道菌群分析方法
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申请号： US10069977申请日： 2000-08-31
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公开(公告)号： US06986991B1公开(公告)日： 2006-01-17
- 发明人: Takakazu Inoue
- 申请人： Takakazu Inoue
- 申请人地址： JP Moriguchi
- 专利权人： Sanyo Electric Co., Ltd.
- 当前专利权人： Sanyo Electric Co., Ltd.
- 当前专利权人地址： JP Moriguchi
- 代理机构： Armstrong, Kratz, Quintos, Hanson & Brooks, LLP
- 优先权： JP11-262590 19990916; JP11-330924 19991122
- 国际申请： PCT/JP00/05943 WO 20000831
- 国际公布： WO01/20032 WO 20010322
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12P19/34
摘要：
A method of analyzing intestinal bacteria flora which comprises: extracting a sample from a subject; extracting bacteria to prepare a bacteria suspension; extracting DNAs of bacteria from the bacteria suspension to prepare a DNA extract liquid; amplifying a specific region such as 16S rDNA using the DNA extract liquid; fractionating the amplified fragments by electrophoresis to obtain a fractional pattern; and comparing the fractional pattern with preliminarily obtained electrophoretic patterns of amplified fragments of intestinal bacteria flora, thereby analyzing the intestinal bacteria flora of the subject.