The present invention provides a method and device for determining the sideband ratio of a superconducting mixer using a comb generator. The method includes the steps of: (a) tentatively adjusting the sideband ratio of a mixer for calibration of an output of the comb generator; (b) determining the signal intensity of a pseudo-celestial signal oscillator for calibration of the output of the comb generator; (c) repeating the steps (a) and (b) to derive the relation between the sideband radio of the mixer for the comb-generator output calibration and the intensity of a pseudo-celestial signal; (d) calibrating the output of the comb generator; and (e) obtaining the sideband ratio of the mixer to be measured.