发明授权
US07038012B1 Enrichment process for H. pylori neutrophil activating protein (NAP) utilizing metal chelate chromatography
失效
使用金属螯合色谱的幽门螺杆菌嗜中性粒细胞活化蛋白（NAP）的富集过程
- 专利标题： Enrichment process for H. pylori neutrophil activating protein (NAP) utilizing metal chelate chromatography
- 专利标题（中）： 使用金属螯合色谱的幽门螺杆菌嗜中性粒细胞活化蛋白（NAP）的富集过程
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申请号： US09647637申请日： 1999-04-07
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公开(公告)号： US07038012B1公开(公告)日： 2006-05-02
- 发明人: Guido Grandi
- 申请人： Guido Grandi
- 申请人地址： IT Siena
- 专利权人： Chiron SRL
- 当前专利权人： Chiron SRL
- 当前专利权人地址： IT Siena
- 代理商 Rebecca M. Hale; Felicity E. Groth; Alisa A. Harbin
- 优先权： GB9807721 19980408
- 国际申请： PCT/IB99/00695 WO 19990407
- 国际公布： WO99/53310 WO 19991021
- 主分类号： C07G7/00
- IPC分类号： C07G7/00 ; A61K39/12
摘要：
The invention relates to a process for enriching the presence of H. pylori NAP in a mixture of proteins. The process involves salting-out other proteins. NAP has been found to remain soluble at ammonium sulphate concentrations of 80% and above. The process may involve the additional step of metal chelate chromatography. The combination of salting-out and metal chelate chromatography results in very pure NAP. The NAP may have the same sequence as NAP naturally occurring in H. pylori and is free from sequences typically associated with recombinant protein production. The processes and NAP of the invention can be used in diagnostic and therapeutic products and processes.
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