发明授权
US07056709B2 Isolation and expression of a gene for a nitrilase from Acidovorax faclilis 72W
失效
从酸性酵母72W中分离和表达腈水解酶基因
- 专利标题： Isolation and expression of a gene for a nitrilase from Acidovorax faclilis 72W
- 专利标题（中）： 从酸性酵母72W中分离和表达腈水解酶基因
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申请号： US10376653申请日： 2003-02-27
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公开(公告)号： US07056709B2公开(公告)日： 2006-06-06
- 发明人: Sarita Chauhan , Robert Dicosimo , Robert D. Fallon , John E. Gavagan , Mark S. Payne
- 申请人： Sarita Chauhan , Robert Dicosimo , Robert D. Fallon , John E. Gavagan , Mark S. Payne
- 申请人地址： US DE Wilmington
- 专利权人： E. I. du Pont de Nemours and Company
- 当前专利权人： E. I. du Pont de Nemours and Company
- 当前专利权人地址： US DE Wilmington
- 代理商 Christine M. Lhulier
- 主分类号： C12P7/40
- IPC分类号： C12P7/40 ; C07H21/04 ; C12Q1/68 ; C07D211/60
摘要：
Recombinant microbial strains are provided that express nitrilase enzyme and are useful as biocatalysts for the hydrolysis of nitrile-containing substrates. The recombinant cells are transformed with a foreign gene isolated from Acidovorax facilis 72W encoding a thermostable nitrilase enzyme that catalyzes the hydrolysis of nitrile-containing substrates to carboxylic acids under mild reaction conditions. The nucleotide sequence of the nitrilase gene and the deduced amino acid sequence encoded by the nitrilase gene are provided.
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