There is disclosed a living tissue harvesting apparatus according to one aspect of the present invention, comprising a sheath which can be inserted in a body through a cut skin portion, an endoscope inserted through the sheath, a cutting device which is disposed at the sheath and which can cut a living tissue, and a holder which is disposed at the sheath to hold a harvesting object tissue in the body and which includes a press discharge portion to press-discharge the harvesting object tissue in a direction detached from the cutting device, wherein the holder includes a hook portion to catch the living tissue in a position disposed opposite to the cutting device, and the cutting device and hook portion can move with respect to each other.