发明授权
- 专利标题: Method for purifying enzymes from clostridium histolyticum using multi-stage chromatography
- 专利标题(中): 使用多级色谱法纯化溶组织梭菌的酶的方法
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申请号: US10478545申请日: 2002-06-01
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公开(公告)号: US07083964B2公开(公告)日: 2006-08-01
- 发明人: Manfred Kurfürst , Stefan Schmidbauer
- 申请人: Manfred Kurfürst , Stefan Schmidbauer
- 申请人地址: DE Uetersen
- 专利权人: Nordmark Arzneimittel GmbH & Co. KG
- 当前专利权人: Nordmark Arzneimittel GmbH & Co. KG
- 当前专利权人地址: DE Uetersen
- 代理商 Friedrich Kueffner
- 优先权: DE10131994 20010702; DE10134347 20010714
- 国际申请: PCT/EP02/05021 WO 20020601
- 国际公布: WO03/004628 WO 20030116
- 主分类号: C12N9/48
- IPC分类号: C12N9/48
摘要:
The invention relates to a method for purifying at least one enzyme obtained in an excess fermentation of Clostridium histolyticum. It is provided for that the enzymes of the excess fermentation are separated by a multistage chromatography method by exclusively using chromatography materials on styrene/divinyl-benzene base and/or on base of in particular ceramic hydroxylapatite.
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