发明授权
US07092078B2 Flow cytometer for classifying leukocytes and method for determining detection angle range of the same
有权
用于分类白细胞的流式细胞仪和确定其检测角度范围的方法
- 专利标题: Flow cytometer for classifying leukocytes and method for determining detection angle range of the same
- 专利标题(中): 用于分类白细胞的流式细胞仪和确定其检测角度范围的方法
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申请号: US10402133申请日: 2003-03-31
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公开(公告)号: US07092078B2公开(公告)日: 2006-08-15
- 发明人: Yutaka Nagai , Kazuo Yamagishi , Tomonobu Maruyama
- 申请人: Yutaka Nagai , Kazuo Yamagishi , Tomonobu Maruyama
- 申请人地址: JP Tokyo
- 专利权人: Nihon Kohden Corporation
- 当前专利权人: Nihon Kohden Corporation
- 当前专利权人地址: JP Tokyo
- 代理机构: Sughrue Mion, PLLC
- 主分类号: G01N33/48
- IPC分类号: G01N33/48
摘要:
A blood sample containing leukocytes flows through a flow cell. A light source emits a light beam in a first direction. The light beam incident into the flow cell is scattered by the blood sample as scattered light. A first detector detects an intensity of forward small scattered light out of the scattered light. The first detector is arranged so as to have a maximum angle of a detection angle range for the forward small scattered light stemmed from the first direction at a position inside (outside) of the flow cell, which is 3.1 (4) degrees or less. A second detector detects an intensity of forward large scattered light out of the scattered light. The second detector is arranged so as to have a detection angle range for the forward large scattered light stemmed from the first direction at a position inside (outside) of the flow cell, which falls in a range from 8 to 12 (10 to 16) degrees. A processor classifies the leukocytes based on the detected intensity of the forward large scattered light and the detected intensity of the forward small scattered light.
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