发明授权
- 专利标题: PCR method
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申请号: US10090326申请日: 2002-03-04
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公开(公告)号: US07101663B2公开(公告)日: 2006-09-05
- 发明人: Tony E. Godfrey , James D. Luketich , Siva Raja , Lori A. Kelly , Sydney D. Finkelstein
- 申请人: Tony E. Godfrey , James D. Luketich , Siva Raja , Lori A. Kelly , Sydney D. Finkelstein
- 申请人地址: US PA Pittsburgh
- 专利权人: University of Pittsburgh—of the Commonwealth System of Higher Education
- 当前专利权人: University of Pittsburgh—of the Commonwealth System of Higher Education
- 当前专利权人地址: US PA Pittsburgh
- 代理商 Jesse A. Hirshman
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12P19/34
摘要:
A method for balancing multiplexed PCR methods is provided. In the method, two or more sequential temporal PCR stages are used to effectively separate two or more PCR reactions in a single tube as an alternative to primer limiting to modulate the relative rate of production of a first amplicon by a first primer set and a second amplicon by a second primer set during the first and second amplification stages. Also provided are rapid RT-PCR methods that find particular use in intraoperative diagnoses and prognoses, for instance in diagnosing malignant esophageal adenocarcenoma by determining expression levels of carcinoembryonic antigen (CEA) in sentinel lymph nodes.
公开/授权文献
- US20030017482A1 PCR method 公开/授权日:2003-01-23