A variable gain amplifying circuit, which applies a resistor ladder to obtain a more precise gain, is disclosed. The amplifying circuit includes an input, an operational amplifier (op-amp), a resistor unit and a feedback resistor. The feedback resistor is coupled between an output and an inverting input of the op-amp. The resistor unit includes at least one resistor ladder. The resistor unit further includes a switching unit for controlling whether the at least one resistor ladder is coupled between the input of the amplifying circuit and the inverting input of the op-amp. A differential amplifying circuit for more precise gain adjustment is also disclosed.