Invention Grant
US07118913B2 Expression vector containing urea cycle enzyme gene, transformant thereof, and use of transformant for protein over-expression
有权
含有尿素循环酶基因的表达载体，其转化体，以及转化体用于蛋白质过表达的用途
- Patent Title: Expression vector containing urea cycle enzyme gene, transformant thereof, and use of transformant for protein over-expression
- Patent Title (中): 含有尿素循环酶基因的表达载体，其转化体，以及转化体用于蛋白质过表达的用途
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Application No.: US10363421Application Date: 2001-08-24
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Publication No.: US07118913B2Publication Date: 2006-10-10
- Inventor: Hong-Jin Kim , Myung-II Chung , Ik-Hwan Kim , Ick-Young Kim
- Applicant: Hong-Jin Kim , Myung-II Chung , Ik-Hwan Kim , Ick-Young Kim
- Applicant Address: KR Seoul
- Assignee: Chung-Ang University Industry Academic Cooperation Foundation
- Current Assignee: Chung-Ang University Industry Academic Cooperation Foundation
- Current Assignee Address: KR Seoul
- Agency: Abelman, Frayne & Schwab
- Priority: KR2000-49688 20000825; KR2001-50083 20010820
- International Application: PCT/KR01/01434 WO 20010824
- International Announcement: WO02/16626 WO 20020228
- Main IPC: C12N5/06
- IPC: C12N5/06 ; C12N15/63
Abstract:
The present invention relates to expression vectors containing in vivo urea cycle enzyme gene; transformants thereof; and the use of transformants for protein overexpression. During the overexpression of protein, if the animal cell transformed with expression vectors of the present invention is used, amounts of ammonia accumulation within cell culture medium will decrease and cell growth rate will increase, thus, it is advantageous to be capable of obtaining desired protein in high yields.
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