The wire spool guide assembly is a conduit through which wire is dispensed in a controlled and orderly manner. The assembly has at least one wire spool guide, a handle bar and a spindle. The spindle may be a single spindle or part of a wire spool cart. The spindle is used to hold wire spools and the handle bar. The handle bar serves to hold the guide. The guide includes an eyebolt rotatably mounted to a support frame via a ball bearing. The eyebolt guides wire as it is dispensed from the spool acquiescing to the direction of the user's pull. Friction, created as wire is pulled through the eyebolt, slows the speed at which the spool rotates on the spindle. The frame of the guide, however, is fixed on the handle bar by a fastener disposed at a bottom end of the guide.