Methods of creating tooling designs for, and the production of, one or more polarizer groups on a workpiece. Each such group includes an optically transmitting substrate with one or more discrete polarizer pixels thereon. The polarizer groups are used with optical instruments having a number of optical channels. In the method, one or more physical characteristics of at least one optical channel of the optical instrument are measured. Based on the determined physical characteristics, one or more discrete polarizer groups to be created on the substrate are defined, by defining for each such group a location on the substrate for the group, the size, shape and location within the group of each polarizer pixel of the group, and the polarization orientation for each such polarizer pixel of the group. The area of each polarizer pixel is then filled with tooling information that will establish its polarization orientation.