发明授权
US07166443B2 Methods, compositions, and automated systems for separating rare cells from fluid samples
有权
用于从流体样品中分离稀有细胞的方法,组合物和自动化系统
- 专利标题: Methods, compositions, and automated systems for separating rare cells from fluid samples
- 专利标题(中): 用于从流体样品中分离稀有细胞的方法,组合物和自动化系统
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申请号: US10701684申请日: 2003-11-04
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公开(公告)号: US07166443B2公开(公告)日: 2007-01-23
- 发明人: George Walker , Junquan Xu , Douglas Yamanishi , Paul Hujsak , Lei Wu , Mingxian Huang , Guoliang Tao , Sara Snyder , Charina Yap
- 申请人: George Walker , Junquan Xu , Douglas Yamanishi , Paul Hujsak , Lei Wu , Mingxian Huang , Guoliang Tao , Sara Snyder , Charina Yap
- 申请人地址: US CA San Diego
- 专利权人: Aviva Biosciences Corporation
- 当前专利权人: Aviva Biosciences Corporation
- 当前专利权人地址: US CA San Diego
- 代理机构: Morrison & Foerster LLP
- 代理商 Kristin Chrudimsky
- 主分类号: C12Q1/04
- IPC分类号: C12Q1/04
摘要:
The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells from a complex fluid sample. In particular, the enrichment of fetal cells from maternal samples, such as maternal blood samples, can greatly aid in the detection of fetal abnormalities or a variety of genetic conditions. In addition, the present invention recognizes that the enrichment of rare malignant cells from patient samples, can aid in diagnosis, prognosis, and development of therapeutic modalities for patients. The invention includes microfabricated filters for filtering fluid samples and methods of enriching rare cells of fluid samples using microfabricated filters of the present invention. The invention also includes solutions for the selective sedimentation of red blood cells (RBCs) from a blood sample and methods of using selective RBC sedimentation solutions for enriching rare cells of a fluid sample. Yet another aspect of the invention is an automated system for processing a fluid sample that includes: at least one filtration chamber that includes a microfabricated filter; automated means for directing fluid flow through at least one filtration chamber of the automated system, and means for collecting enriched rare cells. The present invention also includes methods of using automated systems for separating rare cells from fluid samples. Preferred fluid samples are blood, effusion, or urine samples, and rare cells that can be enriched from such sample include nucleated red blood cells and cancer cells.