发明授权
- 专利标题: Capture and detection format versatility for dipstick assays
- 专利标题(中): 捕捉和检测格式通用性测试仪测试
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申请号: US10332131申请日: 2001-07-06
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公开(公告)号: US07192701B2公开(公告)日: 2007-03-20
- 发明人: Helen Lee , Hsiang Yun Hu , Magda Anastassova Dineva
- 申请人: Helen Lee , Hsiang Yun Hu , Magda Anastassova Dineva
- 申请人地址: US CA Sunnyvale
- 专利权人: Diagnostics for the Real World, Ltd.
- 当前专利权人: Diagnostics for the Real World, Ltd.
- 当前专利权人地址: US CA Sunnyvale
- 代理机构: Proskauer Rose LLP
- 优先权: GB0016833.6 20000707
- 国际申请: PCT/GB01/03029 WO 20010706
- 国际公布: WO02/04669 WO 20020117
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C07H21/02 ; C07H21/04
摘要:
Dipsticks for testing for the presence of a target nucleic acid in a sample solution are described the dipsticks comprise a universal capture probe immobilised at a capture zone of the dipstick. The universal capture probe is capable of hybridising to a hook capture probe which is hybridised to the target nucleic acid in the sample solution. A contact end of the dipstick is contacted with the sample solution to cause hook capture probe hybridised to the target nucleic acid to move by capillary action to the capture zone where the target nucleic acid can be detected. Use of the universal and hook capture probes allows dipsticks to be prepared which can be used to capture any target nucleic acid, thereby simplifying preparation of the dipsticks. Specificity of target nucleic acid capture is then achieved by use of an appropriate hook capture probe. Methods and kits are also described.
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