发明授权
US07270801B2 Fluorogenic or fluorescent reporter molecules and their applications for whole-cell fluorescence screening assays for caspases and other enzymes and the use thereof
失效
荧光或荧光报告分子及其在半胱氨酸蛋白酶和其他酶的全细胞荧光筛选测定中的应用及其用途
- 专利标题： Fluorogenic or fluorescent reporter molecules and their applications for whole-cell fluorescence screening assays for caspases and other enzymes and the use thereof
- 专利标题（中）： 荧光或荧光报告分子及其在半胱氨酸蛋白酶和其他酶的全细胞荧光筛选测定中的应用及其用途
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申请号： US10829381申请日： 2004-04-22
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公开(公告)号： US07270801B2公开(公告)日： 2007-09-18
- 发明人: Eckard Weber , Sui Xiong Cai , John F. W. Keana , John A. Drewe , Han-Zhong Zhang
- 申请人： Eckard Weber , Sui Xiong Cai , John F. W. Keana , John A. Drewe , Han-Zhong Zhang
- 申请人地址： US CA San Diego
- 专利权人： Cytovia, Inc.
- 当前专利权人： Cytovia, Inc.
- 当前专利权人地址： US CA San Diego
- 代理机构： Sterne, Kessler, Goldstein & Fox P.L.L.C.
- 主分类号： A61N49/00
- IPC分类号： A61N49/00
摘要：
The present invention relates to novel fluorescent dyes, novel fluorogenic and fluorescent reporter molecules and new enzyme assay processes that can be used to detect the activity of caspases and other enzymes involved in apoptosis in whole cells, cell lines and tissue samples derived from any living organism or organ. The reporter molecules and assay processes can be used in drug screening procedures to identify compounds which act as inhibitors or inducers of the caspase cascade in whole cells or tissues. The reagents and assays described herein are also useful for determining the chemosensitivity of human cancer cells to treatment with chemotherapeutic drugs. The present invention also relates to novel fluorogenic and fluorescent reporter molecules and new enzyme assay processes that can be used to detect the activity of type 2 methionine aminopeptidase, dipeptidyl peptidase IV, calpain, aminopeptidase, HIV protease, adenovirus protease, HSV-1 protease, HCMV protease and HCV protease.
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