发明授权
US07309571B2 Amplification of self-ligated, circularized cDNA for expression profiling 有权
用于表达谱的自连接的环化cDNA的扩增

  • 专利标题: Amplification of self-ligated, circularized cDNA for expression profiling
  • 专利标题(中): 用于表达谱的自连接的环化cDNA的扩增
  • 申请号: US10770657
    申请日: 2004-02-03
  • 公开(公告)号: US07309571B2
    公开(公告)日: 2007-12-18
  • 发明人: John Nelson
  • 申请人: John Nelson
  • 申请人地址: US NJ Piscataway
  • 专利权人: GE Healthcare Bio-Sciences Corp.
  • 当前专利权人: GE Healthcare Bio-Sciences Corp.
  • 当前专利权人地址: US NJ Piscataway
  • 代理商 Yonggang Ji
  • 主分类号: C12Q1/68
  • IPC分类号: C12Q1/68 C12P19/34
Amplification of self-ligated, circularized cDNA for expression profiling
摘要:
This invention presents a new cDNA amplification method. RNA is first converted to cDNA. The synthesis of cDNA can include a promoter tagged oligonucleotide, and then this cDNA is ligated to form circles (or possibly concatemers). This is then amplified using a Phi29 DNA polymerase based rolling circle and strand displacement amplification. The invention allows for RNA promoter sequences to be attached to the cDNA to facilitate additional amplification through the generation of RNA from the amplified cDNA. The resulting product can then be used to make materials for gene expression studies or other RNA analysis procedures.
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