Embodiments include an imaging system that includes a collimator support base and a detector assembly. The collimator support base is configured to interchangeably accept a slit aperture collimator and a pinhole aperture collimator. The slit aperture collimator has either a corresponding septa assembly or a corresponding crossed-slit collimator. The detector assembly is configured to detect collimated gamma rays emanating from a subject in a field of view of the imaging system and generate one or more signals in response to the detected gamma rays. Methods of adjusting performance of imaging systems are also provided.