发明授权
- 专利标题: High throughput mass spectrometry
- 专利标题(中): 高通量质谱
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申请号: US09502283申请日: 2000-02-11
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公开(公告)号: US07384387B1公开(公告)日: 2008-06-10
- 发明人: Sun Ai Raillard , Yong Hong Chen , Claus Krebber , Jeremy Minshull , Willem P. C. Stemmer
- 申请人: Sun Ai Raillard , Yong Hong Chen , Claus Krebber , Jeremy Minshull , Willem P. C. Stemmer
- 申请人地址: US CA Redwood City
- 专利权人: Maxygen, Inc.
- 当前专利权人: Maxygen, Inc.
- 当前专利权人地址: US CA Redwood City
- 代理商 Sharon M. Fujita; Stacy Landry; Norman J. Kruse
- 主分类号: C40B30/10
- IPC分类号: C40B30/10
摘要:
Apparatus and methods for high throughput mass spectrometry are provided. The methods involve sample preparation in an off-line parallel purification system. Such methods include but are not limited to the use of an appropriate buffer when generating samples or the use of a solid support for tagged components. The samples prepared in this way do not then need to be column separated. The apparatus provided includes a cell growth plate for growing cells and generating products and/or reactants, an off-line parallel purification system, a mass spectrometer, and an automatic sampler that transports samples and injects them into the mass spectrometer of the apparatus. The methods and apparatus described are used, for example, in screening enzyme reaction pathways.