发明授权
- 专利标题： Terminal-phosphate-labeled nucleotides with new linkers
- 专利标题（中）： 末端磷酸酯标记的核苷酸与新的接头
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申请号： US10772996申请日： 2004-02-05
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公开(公告)号： US07393640B2公开(公告)日： 2008-07-01
- 发明人: Shiv Kumar , Mark McDougall , Anup Sood , John Nelson , Carl Fuller , John Macklin , Paul Mitsis
- 申请人： Shiv Kumar , Mark McDougall , Anup Sood , John Nelson , Carl Fuller , John Macklin , Paul Mitsis
- 申请人地址： US NJ Piscataway
- 专利权人： GE Healthcare Bio-Sciences Corp.
- 当前专利权人： GE Healthcare Bio-Sciences Corp.
- 当前专利权人地址： US NJ Piscataway
- 代理商 Yonggang Ji
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12P19/34
摘要：
The present invention describes methods of using terminal-phosphate-labeled nucleotides in the presence of a manganese salt to enhance their substrate properties towards various enzymes. Particularly described are methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases, in the presence of a manganese salt. Further provided are manganese complexes of terminal-phosphate-labeled nucleotides as well as terminal-phosphate-labeled nucleotides with new linkers with enhanced substrate properties.
公开/授权文献
- US20040241716A1 Terminal-phosphate-labeled nucleotides with new linkers 公开/授权日：2004-12-02