发明授权
- 专利标题: Method for the recombinant production and purification of protein kinases
- 专利标题(中): 蛋白激酶重组生产和纯化方法
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申请号: US11000867申请日: 2004-12-01
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公开(公告)号: US07393936B2公开(公告)日: 2008-07-01
- 发明人: Hubert Hertenberger , Konrad Honold , Christian Klein , Petra Rueger
- 申请人: Hubert Hertenberger , Konrad Honold , Christian Klein , Petra Rueger
- 申请人地址: US NJ Nutley
- 专利权人: Hoff Hoffmann-La Roche Inc.
- 当前专利权人: Hoff Hoffmann-La Roche Inc.
- 当前专利权人地址: US NJ Nutley
- 代理商 George W. Johnston; Patricia S. Rocha-Tramaloni
- 优先权: EP03027755 20031202
- 主分类号: C07K1/02
- IPC分类号: C07K1/02 ; C07K1/14 ; C07K1/20 ; C12N9/12 ; C12N15/70
摘要:
A method for the recombinant production and purification of a protein kinase selected from the group consisting of tyrosine protein kinases and serine/threonine kinases by expressing a nucleic acid encoding said kinase in a microbial host cell, forming inclusion bodies containing said kinase, isolating, solubilizing, naturing, and purifying said kinase wherein said purification is performed by hydrophobic interaction with an hydrophobic adsorbent under conditions whereby at least 70% of said protein kinase are not bound to said adsorbent and the protein kinase not bound to said adsorbent is recovered.