A slider separating method includes bonding a row bar containing a plurality of slider-forming portions to a bonding surface of a cutting fixture along a side surface that extends in its length direction; cutting the row bar bonded on the cutting fixture into individual sliders along cutting gaps provided between the slider-forming portions; decreasing bonding force between each diced slider and the bonding surface; and separating the sliders with decreased bonding force from the cutting fixture by pushing each slider along a side direction being parallel to the bonding surface and perpendicular to the length direction and holding and receiving the slider to a slider tray having recesses formed therein which is partitioned according to the sliders. The method can reliably and efficiently separate a row bar containing a plurality of slider-forming portions into individual sliders without depending upon size or arrangement pitch of the sliders and hold these sliders.