发明授权
US07488578B2 Method for detecting DNA point mutations (single nucleotide polymorphism (SNP) analysis) and associated arrangement
有权
DNA点突变检测方法（单核苷酸多态性（SNP）分析）及相关安排
- 专利标题： Method for detecting DNA point mutations (single nucleotide polymorphism (SNP) analysis) and associated arrangement
- 专利标题（中）： DNA点突变检测方法（单核苷酸多态性（SNP）分析）及相关安排
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申请号： US10558709申请日： 2004-05-28
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公开(公告)号： US07488578B2公开(公告)日： 2009-02-10
- 发明人: Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
- 申请人： Walter Gumbrecht , Peter Paulicka , Manfred Stanzel
- 申请人地址： DE Munich
- 专利权人： Siemens Aktiengesellschaft
- 当前专利权人： Siemens Aktiengesellschaft
- 当前专利权人地址： DE Munich
- 代理机构： Harness, Dickey & Pierce, P.L.C.
- 优先权： DE10324912 20030530
- 国际申请： PCT/EP2004/005829 WO 20040528
- 国际公布： WO2004/106546 WO 20041209
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C07H21/02 ; C07H21/04
摘要：
The single nucleotide polymorphism analysis involves the utilization of a DNA hybridization process as well as the use of a DNA chip. A liquid DNA sample to be analyzed is guided over a DNA chip in a defined time course. After successful hybridization, the temperature is modified in a defined manner under low stringency conditions such that scavenger/target DNA hybrids are melted, whereby the melting of the scavenger/target DNA hybrids is detected and evaluated according to the temperature. In addition to the DNA chip, at least one device is provided that controls and regulates the temperature, and another device is provided that controls a lateral flow of liquid against the surface of the DNA chip. Factors for matching hybrids and mismatching/single point mismatching hybrids can be detected and evaluated using appropriate measuring device(s).
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