A method and apparatus for calibrating an apparatus that acquires a sequence of radiographic images and correcting images of an object under observation. For each image of a sequence acquired by the apparatus and for a given frequency of acquisition of the sequence, the apparatus is calibrated by determining the value of the variation of a mean of gray levels in at least one zone of interest of the current image of at least one calibration device, the variation being determined relative to the mean gray level of the first image of the sequence in each zone of interest. The determination of the variation is reiterated for a series of images sequences acquired using calibration devices resulting in first images of mean gray levels different from one sequence to another. Each image of an image sequence of the object under observation is corrected, comprising zones of observation having different gray levels by subtracting from the current image the variation of one gray level relative to the first image of the object, the subtraction being a function of the gray level considered from each zone of observation.