发明授权
- 专利标题: Expression vector encoding a triterpene hydroxylase polypeptide
- 专利标题(中): 编码三萜羟化酶多肽的表达载体
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申请号: US10590661申请日: 2005-02-25
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公开(公告)号: US07575892B2公开(公告)日: 2009-08-18
- 发明人: Hiroaki Hayashi , Kenichiro Inoue , Masateru Hoshino , Masaaki Shibuya , Yutaka Ebizuka
- 申请人: Hiroaki Hayashi , Kenichiro Inoue , Masateru Hoshino , Masaaki Shibuya , Yutaka Ebizuka
- 申请人地址: JP Tokyo
- 专利权人: Meiji Seika Kaisha, Ltd.
- 当前专利权人: Meiji Seika Kaisha, Ltd.
- 当前专利权人地址: JP Tokyo
- 代理机构: Sughrue Mion, PLLC
- 优先权: JP2004-049123 20040225
- 国际申请: PCT/JP2005/003205 WO 20050225
- 国际公布: WO2005/080572 WO 20050901
- 主分类号: C12P33/06
- IPC分类号: C12P33/06 ; C12N15/00 ; C12N1/20 ; C12N1/00 ; C07H21/04 ; C12N9/02
摘要:
Soyasapogenol B is biosynthesized via two steps of hydroxylation reaction of its precursor β-amyrin. However, the gene of the hydroxylase concerned in this reaction has not been revealed. Therefore, it was impossible to apply a genetic engineering technique on the hydroxylase. The present inventors reveals that a sequence which corresponds to a soybean-derived cytochrome P-450 gene CYP93E1 encodes an enzyme protein that carries out hydroxylation of the 24-position of an oleanane type triterpene, and also provides a method for applying said gene making use of a genetic engineering technique.
公开/授权文献
- US20080003639A1 Triterpene Hydroxylase 公开/授权日:2008-01-03